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Effects of NANOparticles on beneficial soil MIcrobes and CROPS (NANOMICROPS)

English title Effects of NANOparticles on beneficial soil MIcrobes and CROPS (NANOMICROPS)
Applicant Bucheli Thomas
Number 131265
Funding scheme NRP 64 Opportunities and Risks of Nanomaterials
Research institution Forschungsanstalt Agroscope Reckenholz-Tänikon ART
Institution of higher education Research Institutes Agroscope - AGS
Main discipline Other disciplines of Environmental Sciences
Start/End 01.05.2011 - 30.06.2015
Approved amount 412'688.00
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All Disciplines (5)

Discipline
Other disciplines of Environmental Sciences
Agricultural and Forestry Sciences
Molecular Biology
Agricultural Engineering
Pedology

Keywords (8)

nanoparticles; soil; wheat; clover; micro-organisms; mycorrhiza; rhizobia; agriculture

Lay Summary (English)

Lead
Lay summary
The addition of synthetic nanoparticles (NP) to formulated plant protection products and fertilizers in agriculture may lead to a reduced use and impact of pesticides and fertilisers in the environment and may increase crop productivity. However, before these advantages can come into play, the risks of nanomaterials for the environment and crops have to be defined to ensure their sustainable and beneficial application.The primary aim of NANOMICROPS is to provide data for risk evaluation of agricultural NP applications. Specifically it will be tested 1) whether NP accumulate in soils, soil microbes, and crops, and 2) whether NP affect microbial population structure and key ecological functions performed by symbiotic soil microbes, in particular nitrogen (N) fixation and phosphorus (P) acquisition, and 3) whether NP affect crop performance, specifically crop yield and quality. Special attention will be paid to two groups of plant root symbionts, i.e. P-supplying ar-buscular mycorrhizal fungi (AMF) and N-fixing bacteria (rhizobia). These beneficial soil microbes are abundant in soil and play key roles in agricultural and natural ecosystems by acquiring limiting nutrients and supporting plant growth. The impact of NP on these soil microbes has not been investigated yet, despite their abundance and ecological importance.NP exposure, fate and effect studies will be performed in experimental settings of increasing complexity, from laboratory cultures over controlled pot experiment to lysimeter studies with real soils. Two agricultural crops, i.e. wheat and clover, and two groups of symbiotic soil microbes, i.e. AMF and rhizobia, will be used. A set of representative agricultural soils will be chosen. Fresh or sterilized soils with or without AMF and rhizobia inoculations will be used. NP which we consider potentially relevant for the agro-environment (e.g., TiO2, Carbon Nanotubes) will be added in increasing concentrations as spray or liquid to crop or soil surfaces, or be mixed homogenously with soil. Several analytical methods will be developed to monitor NP in soil, soil leachate, microbes, and plants. A series of biological endpoints (e.g., microbial biomass, community structure, N-fixation, P-acquisition, crop productivity) will be determined throughout the experiments. These are accompanied by traditional chemical and physical soil parameters to provide essential background information on the systems. Experiments will last from several weeks to full crop cultivation periods.The results of this project will contribute to a better understanding of the fate of NP in agro-environmental systems, and their effects on soil microbes and crop. NANOMICROPS will provide ecotoxicological relevant information on exposure and effects of NP as basis for a comprehensive risk assessment.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
A critical evaluation of nanopesticides and nanofertilizers against their conventional analogues
Kah Melanie, Kookana Rai Singh, Gogos Alexander, Bucheli Thomas Daniel (2018), A critical evaluation of nanopesticides and nanofertilizers against their conventional analogues, in Nature Nanotechnology, 13(8), 677-684.
Effects of titanium dioxide nanoparticles on soil microbial communities and wheat biomass
Moll Janine, Klingenfuss Florian, Widmer Franco, Gogos Alexander, Bucheli Thomas Daniel, van der Heijden Marcel G.A. (2017), Effects of titanium dioxide nanoparticles on soil microbial communities and wheat biomass, in Soil Biology & Biochemistry, 111, 85-93.
What Factors Determine the Retention Behavior of Engineered Nanomaterials in Saturated Porous Media?
Goldberg Eli, McNew Coy, Scheringer Martin, Bucheli Thomas D., Nelson Peter, Hungerbühler Konrad (2017), What Factors Determine the Retention Behavior of Engineered Nanomaterials in Saturated Porous Media?, in Environmental Science & Technology, 51(5), 2729-2737.
Quantification of Carbon Nanotubes in Environmental Matrices: Current Capabilities, Case Studies, and Future Prospects
Petersen Elijah J., Flores-Cervantes D. Xanat, Bucheli Thomas D., Elliott Lindsay C. C., Fagan Jeffrey A., Gogos Alexander, Hanna Shannon, Kägi Ralf, Mansfield Elisabeth, Bustos Antonio R. Montoro, Plata Desiree L., Reipa Vytas, Westerhoff Paul, Winchester Michael R. (2016), Quantification of Carbon Nanotubes in Environmental Matrices: Current Capabilities, Case Studies, and Future Prospects, in Environmental Science & Technology, 50(9), 4587-4605.
Effect of nanoparticles on red clover and its symbiotic microorganisms
Moll J., Gogos A., Bucheli T.D., Widmer F., van der Heijden M.G.A. (2016), Effect of nanoparticles on red clover and its symbiotic microorganisms, in J. Nanobiotechnol., 14, 36.
Effects of titanium dioxide nanoparticles on red clover and its rhizobial symbiont
Moll J., Okupnik A., Gogos A., Knauer K., Bucheli T.D., van der Heijden M.G.A., Widmer F. (2016), Effects of titanium dioxide nanoparticles on red clover and its rhizobial symbiont, in PLOS ONE, 11(5), e0155111.
Vertical transport and plant uptake of nanoparticles in a soil mesocosm experiment
Gogos A., Moll J., Klingenfuss F., van der Heijden M.G.A., Irin F., Green M.J., Zenobi R., Bucheli T.D. (2016), Vertical transport and plant uptake of nanoparticles in a soil mesocosm experiment, in J. Nanobiotechnol., 14, 40.
Prediction of nanoparticle transport behavior from physicochemical properties: machine learning provides insights to guide the next generation of transport models
Goldberg Eli, Scheringer Martin, Bucheli Thomas D., Hungerbühler Konrad (2015), Prediction of nanoparticle transport behavior from physicochemical properties: machine learning provides insights to guide the next generation of transport models, in Environmental Science: Nano, 2(4), 352-360.
Capabilities of asymmetric flow field-flow fractionation coupled to multi-angle light scattering to differentiate between carbon nanotubes and soot in soil
Gogos A., Kaegi R., Zenobi R., Bucheli T.D. (2014), Capabilities of asymmetric flow field-flow fractionation coupled to multi-angle light scattering to differentiate between carbon nanotubes and soot in soil, in Environ. Sci. Nano, 1, 584-594.
Critical assessment of models for transport of engineered nanoparticles in saturated porous media.
Goldberg E., Scheringer M., Bucheli T.D., Hungerbühler K. (2014), Critical assessment of models for transport of engineered nanoparticles in saturated porous media., in Environ. Sci. Technol., 48, 12732-12741.
Potential of hyperspectral imaging microscopy for semi-quantitative analysis of nanoparticle uptake by protozoa
Mortimer M., Gogos A., Bartolome N., Kahru A., Bucheli T.D., Slaveykova V.I. (2014), Potential of hyperspectral imaging microscopy for semi-quantitative analysis of nanoparticle uptake by protozoa, in Environ. Sci. Technol., 48, 8760-8767.
Nano-approaches in plant protection products and fertilizers. Myths, opportunities and risks.
Gogos A., Bucheli T.D., Knauer K. (2013), Nano-approaches in plant protection products and fertilizers. Myths, opportunities and risks., in NewagInternational, March/April, 40-45.
Nanomaterials in plant protection and fertilization: current state, foreseen applications and research priorities
Gogos A., Knauer K., Bucheli T.D (2012), Nanomaterials in plant protection and fertilization: current state, foreseen applications and research priorities, in J. Agric. Food Chem., 60, 9781-9792.

Collaboration

Group / person Country
Types of collaboration
Prof. Vera Slaveykova/University of Geneva Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Thomas Posch/University of Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Prof. Micah J. Green/Texas A&M University United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Prof. Detlef Günther/ETH Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel
Prof. Renato Zenobi/ETH Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Ralf Kägi/Eawag Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Prof. Konrad Hungerbühler/ETH Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
6th Swiss Microbial Ecology Meeting Talk given at a conference Effects of different nanoparticles on soil microbial community structures and plant-microbe interactions 10.09.2015 Ascona, Switzerland van der Heijden Marcel; Widmer Franco; Moll Janine;
SETAC Europe, 24th annual meeting Poster Potential of hyperspectral imaging microscopy for semi-quantitative analysis of nanoparticle uptake into cells 11.05.2014 Basel, Switzerland Bucheli Thomas; Gogos Alexander;
SETAC Europe, 24th annual meeting Poster Effects of TiO2 nanoparticles on the symbiosis of red clover and Rhizobium trifolii in a hydroponic system 11.05.2014 Basel, Switzerland Knauer Katja; Gogos Alexander; Widmer Franco; Bucheli Thomas; van der Heijden Marcel; Moll Janine;
Workshop on “Nanotechnology for the agricultural sector: from research to the field”. European Commission, Joint Research Centre, Institute for Prospective Technological Studies Talk given at a conference Overview of agricultural applications of nanotechnology 21.11.2013 Seville, Spain Gogos Alexander; Bucheli Thomas; Knauer Katja;
NORMAN Working Group N°4 and COST Action ENTER Meeting, Nanoforum 2013 Talk given at a conference Nanomaterials in plant protection products and fertilizers: myth or reality ?! 19.09.2013 Rome, Italy Bucheli Thomas; Knauer Katja; Gogos Alexander;
SETAC Europe, 23th annual meeting Poster Capabilities of aF4-MALS to differentiate Carbon Nanotubes and Soot in natural samples 12.05.2013 Glasgow, Great Britain and Northern Ireland Bucheli Thomas; Gogos Alexander;
SETAC Europe, 23th annual meeting Poster Effects of different TiO2 nanoparticles on rhizobia and their symbiosis with clover 12.05.2013 Glasgow, Great Britain and Northern Ireland Gogos Alexander; Bucheli Thomas; van der Heijden Marcel; Moll Janine; Widmer Franco; Knauer Katja;
The 11th New Ag International Conference and Exhibition Talk given at a conference Nanomaterials in Fertilization: Current State, Foreseen Applications, and Research Priorities 13.03.2013 Rio de Janeiro, Brazil Gogos Alexander; Knauer Katja; Bucheli Thomas;
SETAC Europe, 22th annual meeting Poster Effects of different TiO2 nanoparticles on growth of Rhizobium trifolii in liquid culture 20.05.2012 Berlin, Germany Bucheli Thomas; Moll Janine; Widmer Franco; Gogos Alexander; van der Heijden Marcel;
SETAC Europe, 22th annual meeting Talk given at a conference Nanomaterials in plant protection products: current state, foreseen applications and research priorities 20.05.2012 Berlin, Germany Gogos Alexander; Bucheli Thomas; Knauer Katja;
13th EuCheMS International conference on chemistry and the environment Poster Nanomaterials in the agro-ecosystem – current state, foreseen applications and research priorities 11.09.2011 Zürich, Switzerland Bucheli Thomas; van der Heijden Marcel; Widmer Franco; Gogos Alexander; Knauer Katja;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Ökotoxische Effekte von synthetischen Nanopartikeln, Peak-Vertiefungskurs. Vortragstitel: Effekte von Nanopartikeln auf Pflanzen und nützliche Bodenmikroorganismen Talk 22.04.2015 Dübendorf, Switzerland Moll Janine;


Communication with the public

Communication Title Media Place Year
Media relations: radio, television Nanotechnologie: Viele Chancen – neue Risiken SRF 1, Einstein 21.1.2015 German-speaking Switzerland 2015
Media relations: print media, online media Grosse Diskussionen um winzige Teilchen Die Grüne, 14.11.2013 German-speaking Switzerland 2013
Media relations: print media, online media Nanotechnologie wird Teil des Alltags Schweizer Bauer, 13.3.2013 German-speaking Switzerland 2013
Media relations: print media, online media Nanoteilchen in Badesee und Acker NZZ, 5.6.2013 German-speaking Switzerland 2013
Talks/events/exhibitions Nanopartikel in der Landwirtschaft: Mythos oder Wirklichkeit? German-speaking Switzerland 2012

Abstract

The addition of synthetic nanoparticles (NP) to formulated plant protection products and fertilizers in agriculture may lead to a reduced use and impact of pesticides and fertilisers in the environment and may increase crop productivity. However, before these advantages can come into play, the risks of nanomaterials for the environment and crops have to be defined to ensure their sustainable and beneficial application.The primary aim of NANOMICROPS is to provide data for risk evaluation of agricultural NP applications. Specifically it will be tested 1) whether NP accumulate in soils, soil microbes, and crops, and 2) whether NP affect microbial population structure and key ecological functions performed by symbiotic soil microbes, in particular nitrogen (N) fixation and phosphorus (P) acquisition, and 3) whether NP affect crop performance, specifically crop yield and quality. Special attention will be paid to two groups of plant root symbionts, i.e. P-supplying ar-buscular mycorrhizal fungi (AMF) and N-fixing bacteria (rhizobia). These beneficial soil microbes are abundant in soil and play key roles in agricultural and natural ecosystems by acquiring limiting nutrients and supporting plant growth. The impact of NP on these soil microbes has not been investigated yet, despite their abundance and ecological importance.NP exposure, fate and effect studies will be performed in experimental settings of increasing complexity, from laboratory cultures over controlled pot experiment to lysimeter studies with real soils. Two agricultural crops, i.e. wheat and clover, and two groups of symbiotic soil microbes, i.e. AMF and rhizobia, will be used. A set of representative agricultural soils will be chosen. Fresh or sterilized soils with or without AMF and rhizobia inoculations will be used. NP which we consider potentially relevant for the agro-environment (e.g., TiO2, Carbon Nanotubes) will be added in increasing concentrations as spray or liquid to crop or soil surfaces, or be mixed homogenously with soil. Several analytical methods will be developed to monitor NP in soil, soil leachate, microbes, and plants. A series of biological endpoints (e.g., microbial biomass, community structure, N-fixation, P-acquisition, crop productivity) will be determined throughout the experiments. These are accompanied by traditional chemical and physical soil parameters to provide essential background information on the systems. Experiments will last from several weeks to full crop cultivation periods.The results of this project will contribute to a better understanding of the fate of NP in agro-environmental systems, and their effects on soil microbes and crop. NANOMICROPS will provide ecotoxicological relevant information on exposure and effects of NP as basis for a comprehensive risk assessment.
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