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Pathway Diversity in the Self-Assembly of DNA-Derived Bioconjugates

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Vyborna Yuliia, Vybornyi Mykhailo, Häner Robert,
Project From DNA-Assembled Oligochromophores to Supramolecular Polymers: Aromatic Oligophosphates as Versatile Building Blocks for Functional Materials
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Original article (peer-reviewed)

Journal Bioconjugate Chemistry
Volume (Issue) 27(11)
Page(s) 2755 - 2761
Title of proceedings Bioconjugate Chemistry
DOI 10.1021/acs.bioconjchem.6b00517

Open Access

URL https://boris.unibe.ch/cgi/users/home?screen=EPrint%3A%3AView&eprintid=90082
Type of Open Access Repository (Green Open Access)

Abstract

The pathway diversity of the self-assembly of amphiphilic DNA-pyrene conjugates is described. The hydrophobic pyrene units drive the self-assembly of the anionic oligomers in an aqueous environment into ribbon-shaped, DNA-grafted supramolecular polymers. Isothermal mixing of two types of sorted ribbons, each of which contains only one kind of two complementary oligonucleotides, results in the formation of tight networks. Thermal disassembly of these kinetically trapped networks and subsequent re-assembly of the liberated components leads to mixed supramolecular polymers, which now contain both types of oligonucleotides. The scrambling of the oligonucleotides prevents the interaction between ribbons and, thus, network formation. The results show that a high local density of DNA strands in linear arrays favors hybridization among sorted polymers, whereas hybridization among mixed arrays is prevented. The lack of DNA hybridization among mixed ribbons is ascribed to the electrostatic repulsion between identical, hence non-complementary, oligonucleotides. The findings highlight the importance of kinetically trapped states on the structural and functional properties of supramolecular polymers containing orthogonal self-assembly motifs.
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