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In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2011
Author Tschopp Florence, Charrière Fabien, Schneider André,
Project Mitochondrial biogenesis in T. brucei: import of macromolecules and organellar gene expression
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Original article (peer-reviewed)

Journal EMBO reports
Volume (Issue) 12(8)
Page(s) 825 - 32
Title of proceedings EMBO reports
DOI 10.1038/embor.2011.111


Trypanosoma brucei imports all mitochondrial transfer RNAs (tRNAs) from the cytosol. By using cell lines that allow independent tetracycline-inducible RNA interference and isopropyl-β-D-thiogalactopyranoside-inducible expression of a tagged tRNA, we show that ablation of Tim17 and mitochondrial heat-shock protein 70, components of the inner-membrane protein translocation machinery, strongly inhibits import of newly synthesized tRNAs. These findings, together with previous results in yeast and plants, suggest that the requirement for mitochondrial protein-import factors might be a conserved feature of mitochondrial tRNA import in all systems.