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Functional mapping of yeast genomes by saturated transposition

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Michel Agnès H., Hatakeyama Riko, Kimmig Philipp, Arter Meret, Peter Matthias, Matos Joao, De Virgilio Claudio, Kornmann Benoî T.,
Project ER-phagy mechanisms to maintain and restore endoplasmic reticulum homeostasis
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Original article (peer-reviewed)

Journal eLife
Volume (Issue) 6
Page(s) 1 - 1
Title of proceedings eLife
DOI 10.7554/elife.23570

Abstract

© Michel et al. Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput.
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