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T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays.

Type of publication Not peer-reviewed
Publikationsform Review article (non peer-reviewed)
Publication date 2010
Author Martin Stefan F, Esser Philipp R, Schmucker Sonja, Dietz Lisa, Naisbitt Dean J, Park B Kevin, Vocanson Marc, Nicolas Jean-Francois, Keller Monika, Pichler Werner J, Peiser Matthias, Luch Andreas, Wanner Reinhard, Maggi Enrico, Cavani Andrea, Rustemeyer Thomas, Richter Anne, Thierse Hermann-Josef, Sallusto Federica,
Project Investigating the primary immune response against drugs in humans
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Review article (non peer-reviewed)

Journal Cellular and molecular life sciences : CMLS
Volume (Issue) 67(24)
Page(s) 4171 - 84
Title of proceedings Cellular and molecular life sciences : CMLS
DOI 10.1007/s00018-010-0495-3


Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an overview of the development of the field over the last two decades.