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Transmission Electron Microscopy Imaging to analyse chromatin density distribution at the nanoscale level

Type of publication Peer-reviewed
Publikationsform Contribution to book (peer-reviewed)
Author Fabrice T.N, Cherkezeyan L., Ringli C., Baroux C.,
Project Mechanisms and role of linker histones dynamics in plant reproduction
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Contribution to book (peer-reviewed)

Book Methods in Molecular Biology- Plant Chromatin Dynamics
Editor , Bemer Marian; , Baroux Célia
Publisher Springer, New York
Page(s) 1
ISBN 978-1-4939-7318-7
Title of proceedings Methods in Molecular Biology- Plant Chromatin Dynamics
DOI 10.1007/978-1-4939-7318-7_34


Transmission electron microscopy (TEM) is used to study the fine ultrastructural organization of cells. Delicate specimen preparation is required for results to reflect the “native” ultrastructural organization of sub-cellular features such as the nucleus. Despite the advent of high-resolution, fluorescent imaging of chromatin components, TEM still provides a unique and complementary level of resolution capturing chromatin organisation at the nanoscale level. Here we describe the workflow, from tissue preparation, TEM image acquisition and image processing, for obtaining a quantitative description of chromatin density distribution in plant cells, informing on local fluctuations and periodicity. Comparative analyses then allow to elucidate the structural changes induced by developmental or environmental cues, or by mutations affecting specific chromatin modifiers at the nanoscale level. We argue that this approach remains affordable and merits a renewed interest by the plant chromatin community.