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Lipidated Peptide Dendrimers Killing Multidrug Resistant Bacteria

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Siriwardena Thissa N., StachMichaela, HeRunze, GanBee-Ha, JavorSacha, HeitzMarc, MaLan, CaiXiangju, PengChen, WeiDengwen, LiHongtao, MaJun, KöhlerThilo, van DeldenChristian, DarbreTamis, ReymondJean-Louis,
Project Antimicrobial peptide dendrimers (AMPD) and bicyclic peptides (AMBP) as therapeutic agents against multidrug resistant bacteria
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Original article (peer-reviewed)

Journal Journal of the American Chemical Society
Page(s) 423 - 432
Title of proceedings Journal of the American Chemical Society
DOI 10.1021/jacs.7b11037

Open Access

URL https://pubs.acs.org/doi/10.1021/jacs.7b11037
Type of Open Access Website

Abstract

New antibiotics are urgently needed to address multidrug-resistant (MDR) bacteria. Herein we report that second-generation (G2) peptide dendrimers bearing a fatty acid chain at the dendrimer core efficiently kill Gram-negative bacteria including Pseudomonas aeruginosa and Acinetobacter baumannii, two of the most problematic MDR bacteria worldwide. Our most active dendrimer TNS18 is also active against Gram-positive methicillin-resistant Staphylococcus aureus. Based on circular dichroism and molecular dynamics studies, we hypothesize that TNS18 adopts a hydrophobically collapsed conformation in water with the fatty acid chain backfolded onto the peptide dendrimer branches and that the dendrimer unfolds in contact with the membrane to expose its lipid chain and hydrophobic residues, thereby facilitating membrane disruption leading to rapid bacterial cell death. Dendrimer TNS18 shows promising in vivo activity against MDR clinical isolates of A. baumannii and Escherichia coli, suggesting that lipidated peptide dendrimers might become a new class of antibacterial agents.
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