Back to overview

An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Harner Max E, Unger Ann-Katrin, Geerts Willie JC, Mari Muriel, Izawa Toshiaki, Stenger Maria, Geimer Stefan, Reggiori Fulvio, Westermann Benedikt, Neupert Walter,
Project ER-phagy mechanisms to maintain and restore endoplasmic reticulum homeostasis
Show all

Original article (peer-reviewed)

Journal eLife
Volume (Issue) 5
Page(s) e18853
Title of proceedings eLife
DOI 10.7554/elife.18853

Open Access

Type of Open Access Publisher (Gold Open Access)


Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to the generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the F 1 F O -ATP synthase and the presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution.