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Measurement by SPR of very low dissociation rates: Oxidation-mediated loss of biotin-streptavidin affinity

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author Rebhan Mario A E, Brunschweiger Andreas, Hall Jonathan,
Project Post-transcriptional regulation of germ cell apoptosis in C. elegans
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Original article (peer-reviewed)

Journal ChemBioChem
Volume (Issue) 14(16)
Page(s) 2091 - 2094
Title of proceedings ChemBioChem
DOI 10.1002/cbic.201300468


Long-term relationship: Biotin labels on RNAs, and possibly other biomacromolecules, are easily oxidized causing a dramatic loss of affinity for streptavidin and adversely affecting the measurement of high-affinity interactions. A new SPR method has been developed for measuring the very low rate-dissociation constants of biotin- and biotin oxide-conjugated RNAs with streptavidin. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.