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Image processing of 2D crystal images.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author Arheit Marcel, Castaño-Díez Daniel, Thierry Raphaël, Gipson Bryant R, Zeng Xiangyan, Stahlberg Henning,
Project Software for Electron Microscopy of Membrane Proteins
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Original article (peer-reviewed)

Journal Methods in molecular biology (Clifton, N.J.)
Volume (Issue) 955
Page(s) 171 - 94
Title of proceedings Methods in molecular biology (Clifton, N.J.)
DOI 10.1007/978-1-62703-176-9_10


Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.