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Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Nkot Jeanne Louise, Ngono Bikobo Dominique Serge, Abouem A Zintchem Auguste, Nyemeck Norbert Mbabi, Moni Ndedi Esther Del Florence, Betote Diboué Patrick Hervé, Pegnyemb Dieudonné Emmanuel, Bochet Christian G., Koert Ulrich,
Project Photons in organic synthesis
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Original article (peer-reviewed)

Journal Pharmaceutical Biology
Volume (Issue) 56(1)
Page(s) 318 - 324
Title of proceedings Pharmaceutical Biology
DOI 10.1080/13880209.2018.1476559

Open Access

URL http://doi.org/10.1080/13880209.2018.1476559
Type of Open Access Publisher (Gold Open Access)

Abstract

The roots of Lophira lanceolata Van Tiegh.  Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region.  Even though the MeOH ext. of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown.  Phytochem. investigation of the MeOH root ext. of L. lanceolata and detn. of the antimycobacterial activities of that ext. and constituents against the growth of Mycobacterium tuberculosis.  Column chromatog. was used to provide bioactive phytoconstituents.  Those compds. were elucidated using MS and NMR spectroscopic data.  Antimycobacterial screening of the ext. (4.882-5000 μg/mL in DMSO during 24 h at 37 °C) and isolated compds. (0.244-250 μg/mL in DMSO during 24 h at 37 °C) was performed by microplate alamar blue assay (MABA) against two mycobacterial strains.  The investigation of L. lanceolata MeOH roots ext. provided of mixt. of unsepd. biflavonoids with a newly described one, dihydrolophirone A () assocd. to lophirone A ().  The bioactive compds. that effectively inhibited the growth of M. tuberculosis AC45 were found to be compds. and They exhibited MIC values of 31.25 and 15.75 μg/mL, resp., and their MIC was found to be 62.5 μg/mL against resistant strain AC83.  It is clearly evident from the results obtained that the mycobacterial activity of L. lanceolata could be related mainly to its steroid and flavonoid contents.  Therefore, this study suggests the potential of the above-mentioned classes of compds. as promising candidate agents for developing new anti-tuberculosis drugs.
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