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AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author De Angeli Alexis, Zhang Jingbo, Meyer Stefan, Martinoia Enrico,
Project ABC transporters involved in signalling events
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Original article (peer-reviewed)

Journal Nature Communication
Volume (Issue) 4
Page(s) 1804
Title of proceedings Nature Communication
DOI doi:10.1038/ncomms2815

Open Access

URL http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2815.html
Type of Open Access Publisher (Gold Open Access)

Abstract

Water deficit strongly affects crop productivity. Plants control water loss and CO2 uptake by regulating the aperture of the stomatal pores within the leaf epidermis. Stomata aperture is regulated by the two guard cells forming the pore and changing their size in response to ion uptake and release. While our knowledge about potassium and chloride fluxes across the plasma membrane of guard cells is advanced, little is known about fluxes across the vacuolar membrane. Here we present the molecular identification of the long-sought-after vacuolar chloride channel. AtALMT9 is a chloride channel activated by physiological concentrations of cytosolic malate. Single-channel measurements demonstrate that this activation is due to a malate-dependent increase in the channel open probability. Arabidopsis thaliana atalmt9 knockout mutants exhibited impaired stomatal opening and wilt more slowly than the wild type. Our findings show that AtALMT9 is a vacuolar chloride channel having a major role in controlling stomata aperture.
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