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Rapid and serial quantification of adhesion forces of yeast and mammalian cells

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2012
Author E. Potthoff , O. Guillaume-Gentil , D. Ossola , J. Polesel Maris , S. LeibundGut-Landmann, T. Zambelli, J.A. Vorholt,
Project Force-controlled patch clamp (pc-FluidFM)
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Original article (peer-reviewed)

Journal PLoS ONE
Volume (Issue) 7
Page(s) e52712
Title of proceedings PLoS ONE
DOI 10.1371/journal.pone.0052712

Open Access

Type of Open Access Publisher (Gold Open Access)


Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of adhesion forces of different cell types using a single probe. The pace of single-cell force-spectroscopy was accelerated to up to 200 yeast and 20 mammalian cells per probe when replacing the conventional cell trapping cantilever chemistry of atomic force microscopy by underpressure immobilization with fluidic force microscopy (FluidFM). In consequence, statistically relevant data could be recorded in a rapid manner, the spectrum of examinable cells was enlarged, and the cell physiology preserved until approached for force spectroscopy. Adhesion forces of Candida albicans increased from below 4 up to 16 nN at 37°C on hydrophobic surfaces, whereas a Δhgc1-mutant showed forces consistently below 4 nN. Monitoring adhesion of mammalian cells revealed mean adhesion forces of 600 nN of HeLa cells on fibronectin and were one order of magnitude higher than those observed for HEK cells.