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Green light for quantitative live-cell imaging in plants.

Type of publication Peer-reviewed
Publikationsform Review article (peer-reviewed)
Author Grossman G, Krebs M, Maizel A, Stahl Y, Vermeer JE, OttT,
Project Talking with the neighbours: Understanding spatial accommodation during plant development
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Review article (peer-reviewed)

Journal Journal of Cell Science
Volume (Issue) 131(2)
Page(s) 1 - 14
Title of proceedings Journal of Cell Science

Abstract

Plants exhibit an intriguing morphological and physiological plasticity that enables them to thrive in a wide range of environments. To understand the cell biological basis of this unparalleled competence, a number of methodologies have been adapted or developed over the last decades that allow minimal or non-invasive live cell imaging in the context of tissues. Combined with the ease to generate transgenic reporter lines in specific genetic backgrounds or accessions, we are witnessing a blooming in plant cell biology. However, the imaging of plant cells entails a number of specific challenges, such as high levels of autofluorescence, light scattering that is caused by cell walls, or their sensitivity to environmental conditions. Quantitative live-cell imaging in plants therefore requires adapting or developing imaging techniques, as well as mounting and incubation systems, such as micro-fluidics. Here, we discuss some of these obstacles and review a number of selected state-of-the-art techniques, such as two-photon imaging, light sheet microscopy and variable angle epifluorescence microscopy that allow high performance and minimal invasive live-cell imaging in plants.
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