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Sequence determinants of a microtubule tip localization signal (MtLS)

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2012
Author Buey R.M., Sen I., Kortt O., Mohan R., Gfeller D., Veprintsev D., Kretzschmar I., Scheuermann J., Neri D., Zoete V., Michielin O., de Pereda J.M., Akhmanova A., Volkmer R., Steinmetz M.O.,
Project Protein interactions regulating the microtubule cytoskeleton
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Original article (peer-reviewed)

Journal J. Biol. Chem.
Volume (Issue) 287(34)
Page(s) 28227 - 28242
Title of proceedings J. Biol. Chem.
DOI doi: 10.1074/jbc.M112.373928

Abstract

Microtubule plus-end tracking proteins (+TIPs) specifically localize to the growing plus ends of microtubules to regulate microtubule dynamics and functions. A large group of +TIPs contain a short linear motif, SxIP, which is essential for them to bind to EB proteins and target microtubule ends. The SxIP sequence site thus acts as a widespread microtubule tip localization signal (MtLS). Here we have analyzed the sequence-function relationship of a canonical MtLS. Using synthetic peptide arrays on membrane supports, we identified the residue preferences at each amino acid position of the SxIP motif and its surrounding sequence with respect to EB binding. We further developed an assay based on fluorescence polarization to assess the mechanism of the EB-SxIP interaction and to correlate EB-binding and microtubule tip tracking of MtLS sequences from different +TIPs. Finally, we investigated the role of phosphorylation in regulating the EB-SxIP interaction. Together, our results define the sequence determinants of a canonical MtLS and provide the experimental data for bioinformatics approaches to carry out genome-wide predictions of novel +TIPs in multiple organisms
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