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Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Sánchez-Martín Javier, Widrig Victoria, Herren Gerhard, Wicker Thomas, Zbinden Helen, Gronnier Julien, Spörri Laurin, Praz Coraline R., Heuberger Matthias, Kolodziej Markus C., Isaksson Jonatan, Steuernagel Burkhard, Karafiátová Miroslava, Doležel Jaroslav, Zipfel Cyril, Keller Beat,
Project Molecular analysis of disease resistance specificity in cereals
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Original article (peer-reviewed)

Journal Nature Plants
Volume (Issue) 7(3)
Page(s) 327 - 341
Title of proceedings Nature Plants
DOI 10.1038/s41477-021-00869-2

Open Access

URL https://www.nature.com/articles/s41477-021-00869-2
Type of Open Access Repository (Green Open Access)

Abstract

Crop breeding for resistance to pathogens largely relies on genes encoding receptors that confer race-specific immunity. Here, we report the identification of the wheat Pm4 race-specific resistance gene to powdery mildew. Pm4 encodes a putative chimeric protein of a serine/threonine kinase and multiple C2 domains and transmembrane regions, a unique domain architecture among known resistance proteins. Pm4 undergoes constitutive alternative splicing, generating two isoforms with different protein domain topologies that are both essential for resistance function. Both isoforms interact and localize to the endoplasmatic reticulum when co-expressed. Pm4 reveals additional diversity of immune receptor architecture to be explored for breeding and suggests an endoplasmatic reticulum-based molecular mechanism of Pm4-mediated race-specific resistance.
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