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Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Fytianos K., Rodriguez-Lorenzo L., Clift M.J., Blank F., Vanhecke D., von Garnier C., Petri-Fink A., Rothen-Rutishauser B.,
Project Biomedical nanoparticles as immune-modulators
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Original article (peer-reviewed)

Journal Nanomedicine: NBM
Title of proceedings Nanomedicine: NBM

Abstract

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant Tumor Necrosis Factor-alpha release was induced. In contrast, (PEG+PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused Interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties
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