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Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Kwok A., Eggimann G. A., Heitz M., Reymond Jean-Louis, Hollfelder F., Darbre T.,
Project A Chemical Space Approach to Bioactive Peptides
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Original article (peer-reviewed)

Journal ChemBioChem Communications
Volume (Issue) 17
Page(s) 2223 - 2229
Title of proceedings ChemBioChem Communications
DOI 10.1002/cbic.201600485

Open Access

Abstract

Efficient delivery of small interfering RNA (siRNA) into cells is the basis of target‐gene‐specific silencing and, ultimately, gene therapy. However, current transfection reagents are relatively inefficient, and very few studies provide the sort of systematic understanding based on structure–activity relationships that would provide rationales for their improvement. This work established peptide dendrimers (administered with cationic lipids) as siRNA transfection reagents and recorded structure–activity relationships that highlighted the importance of positive charge distribution in the two outer layers and a hydrophobic core as key features for efficient performance. These dendrimer‐based transfection reagents work as well as highly optimised commercial reagents, yet show less toxicity and fewer off‐target effects. Additionally, the degrees of freedom in the synthetic procedure will allow the placing of decisive recognition features to enhance and fine‐tune transfection and cell specificity in the future.
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