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Lysophosphatidic Acid-Mediated GPR35 Signaling in CX3CR1+ Macrophages Regulates Intestinal Homeostasis

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Kaya Berna, Doñas Cristian, Wuggenig Philipp, Diaz Oscar E., Morales Rodrigo A., Melhem Hassan, Hernández Pedro P., Kaymak Tanay, Das Srustidhar, Hruz Petr, Franc Yannick, Geier Florian, Ayata C. Korcan, Villablanca Eduardo J., Niess Jan Hendrik,
Project The Role of IL-20 Cytokine Family Members in Eosinophilic Esophagitis
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Original article (peer-reviewed)

Journal Cell Reports
Volume (Issue) 32(5)
Page(s) 107979 - 107979
Title of proceedings Cell Reports
DOI 10.1016/j.celrep.2020.107979

Open Access

URL http://doi.org/10.1016/j.celrep.2020.107979
Type of Open Access Publisher (Gold Open Access)

Abstract

Single-nucleotide polymorphisms in the gene encoding G protein-coupled receptor 35 (GPR35) are associated with increased risk of inflammatory bowel disease. However, the mechanisms by which GPR35 modulates intestinal immune homeostasis remain undefined. Here, integrating zebrafish and mouse experimental models, we demonstrate that intestinal Gpr35 expression is microbiota dependent and enhanced upon inflammation. Moreover, murine GPR35+ colonic macrophages are characterized by enhanced production of pro-inflammatory cytokines. We identify lysophosphatidic acid (LPA) as a potential endogenous ligand produced during intestinal inflammation, acting through GPR35 to induce tumor necrosis factor (Tnf) expression in macrophages. Mice lacking Gpr35 in CX3CR1+ macrophages aggravate colitis when exposed to dextran sodium sulfate, which is associated with decreased transcript levels of the corticosterone-generating gene Cyp11b1 and macrophage-derived Tnf. Administration of TNF in these mice restores Cyp11b1 expression and intestinal corticosterone production and ameliorates DSS-induced colitis. Our findings indicate that LPA signals through GPR35 in CX3CR1+ macrophages to maintain TNF-mediated intestinal homeostasis.
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