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OMIP-047: High-Dimensional phenotypic characterization of B cellsOMIP-047

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Liechti Thomas, Günthard Huldrych F., Trkola Alexandra,
Project The role of humoral immunity in HIV infection - Understanding broadly neutralizing antibody evolution
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Original article (peer-reviewed)

Journal Cytometry Part A
Volume (Issue) 93(6)
Page(s) 592 - 596
Title of proceedings Cytometry Part A
DOI 10.1002/cyto.a.23488

Open Access

URL https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.23488
Type of Open Access Publisher (Gold Open Access)

Abstract

This 16‐color, 18‐parameter panel was designed to allow a detailed dissection of human B cell subsets and their phenotype in peripheral blood mononuclear cells (PBMC) in healthy donors and in the context of chronic viral diseases such as Human Immunodeficiency Virus 1 (HIV‐1) infection. The panel encompasses a range of backbone markers for the accurate definition of common B cell subsets with a focus on memory B cells and a unique collection of phenotypic markers (chemokine receptors, cytokine receptor, B cell receptor isotypes, and proliferation marker) not combined in multicolor flow cytometry B cell phenotyping thus far. This new panel allows highly detailed phenotypic and functional investigations of B cell subsets. The panel was validated using cryopreserved PBMC from healthy and HIV‐1 infected donors allowing the retrospective analysis of clinical samples
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