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Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Sánchez-Martín Javier, Steuernagel Burkhard, Ghosh Sreya, Herren Gerhard, Hurni Severine, Adamski Nikolai, Vrána Jan, Kubaláková Marie, Krattinger Simon G., Wicker Thomas, Doležel Jaroslav, Keller Beat, Wulff Brande B. H.,
Project Molecular analysis of three different types of fungal disease resistance mechanisms in cereals
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Original article (peer-reviewed)

Journal Genome Biology
Volume (Issue) 17(1)
Page(s) 221 - 221
Title of proceedings Genome Biology
DOI 10.1186/s13059-016-1082-1

Open Access

Abstract

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
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