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Systems-level interference strategies to decipher host factors involved in bacterial pathogen interaction: from RNAi to CRISPRi.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Québatte Maxime, Dehio Christoph,
Project Bacterial Type IV Secretion (T4S): Cellular, Molecular, and Evolutionary Basis of the Subversion of Host Cell Functions by Translocated Effector Proteins
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Original article (peer-reviewed)

Journal Current opinion in microbiology
Volume (Issue) 39
Page(s) 34 - 41
Title of proceedings Current opinion in microbiology
DOI 10.1016/j.mib.2017.08.002

Open Access

Abstract

Bacterial pathogen-host cell interactions involve an intricate interplay of multiple components from both partners. Systems level surveys have been used widely to profile host requirements for pathogen infection. Functional genomics, and more specifically genome-wide perturbation screens, constitute attractive methodologies to assess such host infectomes. Although these strategies have successfully identified numerous critical host factors, they may have failed in generating the high-quality data required for systems level analysis. This is the case for most RNA interference (RNAi) setups with their high propensity to off-target effects (OTE). However, recent efforts to circumvent OTE in RNAi-based experiments as well as the emergence of alternative strategies will likely soon allow significant breakthrough in the systems level understanding of bacterial pathogen-host interactions.
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