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Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2012
Author Mulet Magdalena, Gomila Margarita, Lemaitre Bruno, Lalucat Jorge, García-Valdés Elena,
Project The Drosophila intestinal epithelium: an immune barrier
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Original article (peer-reviewed)

Journal Systematic and applied microbiology
Volume (Issue) 35(3)
Page(s) 145 - 9
Title of proceedings Systematic and applied microbiology
DOI 10.1016/j.syapm.2011.12.003


An entomopathogenic, Gram-negative bacterium isolated from a female specimen of the fruit fly Drosophila melanogaster was taxonomically characterised. Strain L48(T) was strictly aerobic, non-fermentative, oxidase and catalase positive, rod-shaped, and motile due to a polar inserted flagellum. Phylogenetic analysis of the 16S rRNA gene and three other housekeeping genes placed strain L48 (T) in the Pseudomonas putida phylogenetic group. DNA-DNA hybridisation studies together with phenotypic metabolic tests and MALDI-TOF MS analysis justified the proposal of strain L48(T) as a representative of a novel species, for which the name Pseudomonas entomophila sp. nov. is proposed. The type strain is deposited in culture collections under accession numbers CCUG 61470(T) and CECT 7985(T).