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Lipidated Peptide Dendrimers Killing Multidrug-Resistant Bacteria
Type of publication
Peer-reviewed
Publikationsform
Original article (peer-reviewed)
Author
Siriwardena Thissa N., Stach Michaela, He Runze, Gan Bee Ha, Javor Sacha, Heitz Marc, Ma Lan, Cai Xiangjun, Chen Peng, Wei Dengwen, Li Hongtao, Ma Jun, Koehler Thilo, van Delden Christian, Darbre Tamis, Reymond Jean-Louis,
Project
A Chemical Space Approach to Bioactive Peptides
Show all
Original article (peer-reviewed)
Journal
JACS, Journal of the American Chemical Society
Volume (Issue)
140(1)
Page(s)
423 - 432
Title of proceedings
JACS, Journal of the American Chemical Society
DOI
10.1021/jacs.7b11037
Open Access
URL
https://pubs.acs.org/doi/pdf/10.1021/jacs.7b11037
Type of Open Access
Publisher (Gold Open Access)
Abstract
New antibiotics are urgently needed to address multidrug-resistant (MDR) bacteria. Herein we report that second-generation (G2) peptide dendrimers bearing a fatty acid chain at the dendrimer core efficiently kill Gram-negative bacteria including Pseudomonas aeruginosa and Acinetobacter baumannii, two of the most problematic MDR bacteria worldwide. Our most active dendrimer TNS18 is also active against Gram-positive methicillin-resistant Staphylococcus aureus. Based on circular dichroism and molecular dynamics studies, we hypothesize that TNS18 adopts a hydrophobically collapsed conformation in water with the fatty acid chain backfolded onto the peptide dendrimer branches and that the dendrimer unfolds in contact with the membrane to expose its lipid chain and hydrophobic residues, thereby facilitating membrane disruption leading to rapid bacterial cell death. Dendrimer TNS18 shows promising in vivo activity against MDR clinical isolates of A. baumannii and Escherichia coli, suggesting that lipidated peptide dendrimers might become a new class of antibacterial agents.
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