Publication

Back to overview

Measuring in vivo protein half-life.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2011
Author Bojkowska Karolina, Santoni de Sio Francesca, Barde Isabelle, Offner Sandra, Verp Sonia, Heinis Christian, Johnsson Kai, Trono Didier,
Project Innate defenses against retroelements
Show all

Original article (peer-reviewed)

Journal Chemistry & biology
Volume (Issue) 18(6)
Page(s) 805 - 15
Title of proceedings Chemistry & biology
DOI 10.1016/j.chembiol.2011.03.014

Abstract

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.
-