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SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Lindner Heike, Raissig Michael T, Sailer Christian, Shimosato-Asano Hiroko, Bruggmann Rémy, Grossniklaus Ueli,
Project The genetic and molecular basis of gametogenesis and maternal effects in arabidopsis
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Original article (peer-reviewed)

Journal Genetics
Volume (Issue) 191(4)
Page(s) 1381 - 6
Title of proceedings Genetics
DOI 10.1534/genetics.112.141341

Abstract

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.
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