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Facile and rapid formation of giant vesicles from glass beads

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Tanasescu Radu, Mettal Ute, Colom Adai, Colom Adai, Roux Aurélien, Roux Aurélien, Zumbuehl Andreas, Zumbuehl Andreas,
Project Exploring Stable Phospholipid Membranes
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Original article (peer-reviewed)

Journal Polymers
Volume (Issue) 10(1)
Page(s) 54
Title of proceedings Polymers
DOI 10.3390/polym10010054

Abstract

© 2018 by the authors. Giant vesicles (GVs) are widely-usedmodel systems for biologicalmembranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
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