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Polymerase incorporation of pyrene-nucleoside triphosphates

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Hollenstein Marcel, Wojciechowski Filip, Leumann Christian,
Project Improving the catalytic repertoire of DNA enzymes
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Original article (peer-reviewed)

Journal Bioorg. Med. Chem. Lett.
Volume (Issue) 22
Page(s) 4428 - 4430
Title of proceedings Bioorg. Med. Chem. Lett.


Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.