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Merging of image data in electron crystallography.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author Arheit Marcel, Castaño-Diéz Daniel, Thierry Raphaël, Abeyrathne Priyanka, Gipson Bryant R, Stahlberg Henning,
Project Software for Electron Microscopy of Membrane Proteins
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Original article (peer-reviewed)

Journal Methods in molecular biology (Clifton, N.J.)
Volume (Issue) 955
Page(s) 195 - 209
Title of proceedings Methods in molecular biology (Clifton, N.J.)
DOI 10.1007/978-1-62703-176-9_11

Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.
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