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Improved in vivo two-photon imaging after blood replacement by perfluorocarbon

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Haiss F., Jolivet R., Wyss M. T., Reichold J., Braham N. B., Scheffold F., Krafft M. P., Weber B.,
Project A multimodal approach to cerebral blood flow and metabolism
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Original article (peer-reviewed)

Journal The Journal of Physiology
Volume (Issue) 587
Page(s) 3153 - 8
Title of proceedings The Journal of Physiology


Two-photon microscopy is a powerful method in biomedical research that allows functional and anatomical imaging at a subcellular resolution in vivo. The technique is seriously hampered by absorption and scattering of light by blood, which prevents imaging through large vessels. Here, we demonstrate in the rat cerebral cortex that blood replacement by perfluorocarbon emulsion, a compound also used in human critical care medicine, yields superior image quality, while preserving neuronal integrity. Shadows of large superficial vessels disappear completely and cells can be imaged underneath them. For the first time, it is possible to image complete populations of neurons and astrocytes in the upper layers of neocortex in vivo.