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Automation of image processing in electron crystallography.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author Arheit Marcel, Castaño-Díez Daniel, Thierry Raphaël, Gipson Bryant R, Zeng Xiangyan, Stahlberg Henning,
Project Software for Electron Microscopy of Membrane Proteins
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Original article (peer-reviewed)

Journal Methods in molecular biology (Clifton, N.J.)
Volume (Issue) 955
Page(s) 313 - 30
Title of proceedings Methods in molecular biology (Clifton, N.J.)
DOI 10.1007/978-1-62703-176-9_18


Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D crystals have to be processed. Certain processing steps are thereby similar for batches of images that were recorded under similar conditions. Here we describe how the 2dx software package can be used to automate the processing of 2D crystal images, and how the 2D and 3D merging results can be used to iteratively reprocess the images. While the processing of 2D crystal images has been fully automated, the merging process is still semi-manual.