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Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4(+) T Cell Proliferation.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Seydoux Emilie, Rodriguez-Lorenzo Laura, Blom Rebecca A M, Stumbles Philip A, Petri-Fink Alke, Rothen-Rutishauser Barbara M, Blank Fabian, von Garnier Christophe,
Project Biomedical nanoparticles as immune-modulators
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Original article (peer-reviewed)

Journal Nanomedicine : nanotechnology, biology, and medicine
Volume (Issue) 12(7)
Page(s) 1815 - 1826
Title of proceedings Nanomedicine : nanotechnology, biology, and medicine
DOI 10.1016/j.nano.2016.02.020

Abstract

To address how surface charge affects the fate of potential nanocarriers in the lung, gold nanoparticles (AuNPs) coated with polyvinyl alcohol containing either positively (NH2) or negatively (COOH) charged functional groups were intra-nasally instilled in mice, and their uptake by antigen presenting cell populations (APC) in broncho-alveolar lavage (BAL) fluid, trachea, and lung parenchyma, as well as trafficking to the lung draining lymph nodes (LDLNs) was assessed by flow cytometry. Furthermore, CD4(+) T cell proliferation in LDLNs was investigated following instillation. All APC subpopulations preferentially captured positively-charged AuNPs compared to their negatively-charged counterparts. Uptake of AuNPs up-regulated expression of co-stimulatory molecules on all APC populations. Furthermore, positively-charged AuNPs induced enhanced OVA-specific CD4(+) T cell stimulation in LDLNs compared to negatively-charged AuNPs, or polymer alone. Our findings demonstrate surface charge as a key parameter determining particle uptake by APC, and down-stream immune responses depend on the presence of particle core-bound polymer.
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