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Molecular Analyses Define Vα7.2-Jα33+ MAIT Cell Depletion in HIV Infection: A Case-Control Study.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Ussher James E, Phalora Prabhjeet, Cosgrove Cormac, Hannaway Rachel F, Rauch Andri, Günthard Huldrych F, Goulder Philip, Phillips Rodney E, Willberg Christian B, Klenerman Paul,
Project Swiss HIV Cohort Study (SHCS)
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Original article (peer-reviewed)

Journal Medicine
Volume (Issue) 94(29)
Page(s) 1134 - 1134
Title of proceedings Medicine
DOI 10.1097/md.0000000000001134

Open Access

URL https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26200614/
Type of Open Access Publisher (Gold Open Access)

Abstract

Mucosal-associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection. In this case-control study of HIV-positive patients and healthy controls, quantitative real-time polymerase chain reaction was used to assess the abundance of messenger RNA (mRNA) and genomic DNA (gDNA) encoding the canonical MAIT cell T cell receptor (Vα7.2-Jα33). Comparison was made with flow cytometry. Significant depletion of both Vα7.2-Jα33 mRNA and gDNA was seen in HIV infection. Depletion of Vα7.2+CD161++ T cells was confirmed by flow cytometry. In HIV infection, the abundance of Vα7.2-Jα33 mRNA correlated most strongly with the frequency of Vα7.2+CD161++ cells. No increase was observed in the frequency of Vα7.2+CD161- cells among CD3+CD4- lymphocytes. MAIT cells are depleted from blood in HIV infection as confirmed by independent assays. Significant accumulation of a CD161- MAIT cell population is unlikely. Molecular approaches represent a suitable alternative to flow cytometry-based assays for tracking of MAIT cells in HIV and other settings.
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