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Reprogramming of TLR7 signaling enhances anti- tumor NK and cytotoxic T cell responses.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Hotz C Treinies M Mottas I Rötzer L Oberson A Perdicchio M Spagnuolo L Spinetti T Herbst T ,
Project ProDoc Cell Migration Research Module 3: Soluble factors in Cell Migration
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Original article (peer-reviewed)

Journal Oncoimmunology
Volume (Issue) 5(11)
Page(s) e1232219
Title of proceedings Oncoimmunology

Open Access

URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5139637/
Type of Open Access Publisher (Gold Open Access)

Abstract

Toll-like receptor (TLR) 7 agonists are effective in topical application for the immunotherapy of skin cancers, but their performance for the systemic treatment of solid tumors is limited by the development of TLR tolerance. In this study, we describe a novel strategy to overcome TLR tolerance and enhance TLR7-dependent antitumor immune responses through reprogramming of TLR signaling pathways. The sensitivity of TLR7 signaling in dendritic cells (DC) was increased by prior stimulation with the dsRNA poly(I:C) that mimics virally induced immune activation. Timing of the stimulations was important, as sequential stimulation with poly(I:C) and the TLR7 agonist R848 interspaced by 24 h induced higher MAPK and NFkB signaling in DC than the simultaneous application of the same ligands. DC activated by sequential poly(I:C)/R848 stimulation efficiently induced Th1 differentiation and primed NK-cell and cytotoxic T-cell responses. We have developed a treatment regimen taking advantage of TLR7 reprogram-ming that cured over 80% of large immunogenic tumors in mice by the action of NK cells and cytotoxic T cells. These results have direct implications for the use of these clinically established ligands in the immunotherapy of cancer.
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