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A cell biological view of the siderophore pyochelin iron uptake pathway in Pseudomonas aeruginosa

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Cunrath Olivier, Gasser Véronique, Hoegy Françoise, Reimmann Cornelia, Guillon Laurent, Schalk Isabelle J.,
Project Enantio-pyochelin: Biosynthesis, regulation, and natural occurrence
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Original article (peer-reviewed)

Journal Environmental Microbiology
Title of proceedings Environmental Microbiology
DOI 10.1111/1462-2920.12544

Open Access

Abstract

Pyochelin (PCH) is a siderophore produced and secreted by Pseudomonas aeruginosa for iron capture. Using 55 Fe uptake and binding assays, we showed that PCH-Fe uptake in P. aeruginosa involves, in addition to the highly studied outer membrane transporter FptA, the inner membrane permease FptX, which recognizes PCH-55 Fe with an affinity of 0.6 ± 0.2 nM and transports the ferri-siderophore complex from the periplasm into the cytoplasm: fptX deletion inhibited 55 Fe accumulation in the bacterial cytoplasm. Chromosomal replacement was used to generate P. aeruginosa strains producing fluorescent fusions with FptX, PchR (an AraC regulator), PchA (the first enzyme involved in the PCH biosynthesis) and PchE (a non-ribosomic peptide-synthetase involved in a further step). Fluorescence imaging and cellular fractionation showed a uniform repartition of FptX in the inner membrane. PchA and PchE were found in the cytoplasm, associated to the inner membrane all over the bacteria and also concentrated at the bacterial poles. PchE clustering at the bacterial poles was dependent on PchA expression, but on the opposite PchA clustering and membrane association was PchE-independent. PchA and PchE cellular organization suggests the existence of a siderosome for PCH biosynthesis as previously proposed for pyoverdine biosynthesis (another siderophore produced by P. aeruginosa).
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