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CD31 (PECAM-1) Serves as the Endothelial Cell-Specific Receptor of Clostridium perfringens β-Toxin

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Bruggisser Julia, Tarek Basma, Wyder Marianne, Müller Philipp, von Ballmoos Christoph, Witz Guillaume, Enzmann Gaby, Deutsch Urban, Engelhardt Britta, Posthaus Horst,
Project Functional investigations of bacterial and eukaryotic membrane proteins
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Original article (peer-reviewed)

Journal Cell Host {&} Microbe
Publisher Elsevier {BV}
Volume (Issue) 28(1)
Page(s) 69
Title of proceedings Cell Host {&} Microbe
DOI 10.1016/j.chom.2020.05.003

Open Access

Type of Open Access Repository (Green Open Access)


Clostridium perfringens b-toxin (CPB) is a highly active b-pore-forming toxin (b-PFT) and the essential virulence factor for fatal, necro-hemorrhagic enteritis in animals and humans. The molecular mechanisms involved in CPB’s action on its target, the endothelium of small intestinal vessels, are poorly understood. Here, we identify platelet endothelial cell adhesion molecule-1 (CD31 or PECAM-1) as the specific membrane receptor for CPB on endothelial cells. CD31 expression corresponds with the cell-type specificity of CPB, andit is essential for toxicity in cultured cells and mice. Ectopic CD31 expression renders resistant cells and liposomes susceptible to CPB-induced membrane damage. Moreover, the extracellular Ig6 domain of mouse, human, and porcine CD31 is essential for the interaction with CPB. Hence, our results explain the cell-type specificity of CPB in vitro and in the natural disease caused by C. perfringens type C.