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Nitrogen isotope effects can be used to diagnose N transformations in wastewater anammox systems

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Magyar Paul M., Hausherr Damian, Niederdorfer Robert, Stöcklin Nicolas, Wei Jing, Mohn Joachim, Bürgmann Helmut, Joss Adriano, Lehmann Moritz F.,
Project Advanced understanding of autotrophic nitrogen removal and associated N2O emissions in mixed nitritation-anammox systems through combined stable ISOtopic and MOLecular constraints (ISOMOL)
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Original article (peer-reviewed)

Journal Scientific Reports
Volume (Issue) 11(1)
Page(s) 7850 - 7850
Title of proceedings Scientific Reports
DOI 10.1038/s41598-021-87184-0

Open Access

URL http://doi.org/10.1038/s41598-021-87184-0
Type of Open Access Publisher (Gold Open Access)

Abstract

Abstract Anaerobic ammonium oxidation (anammox) plays an important role in aquatic systems as a sink of bioavailable nitrogen (N), and in engineered processes by removing ammonium from wastewater. The isotope effects anammox imparts in the N isotope signatures ( 15 N/ 14 N) of ammonium, nitrite, and nitrate can be used to estimate its role in environmental settings, to describe physiological and ecological variations in the anammox process, and possibly to optimize anammox-based wastewater treatment. We measured the stable N-isotope composition of ammonium, nitrite, and nitrate in wastewater cultivations of anammox bacteria. We find that the N isotope enrichment factor 15 ε for the reduction of nitrite to N 2 is consistent across all experimental conditions (13.5‰ ± 3.7‰), suggesting it reflects the composition of the anammox bacteria community. Values of 15 ε for the oxidation of nitrite to nitrate (inverse isotope effect, − 16 to − 43‰) and for the reduction of ammonium to N 2 (normal isotope effect, 19–32‰) are more variable, and likely controlled by experimental conditions. We argue that the variations in the isotope effects can be tied to the metabolism and physiology of anammox bacteria, and that the broad range of isotope effects observed for anammox introduces complications for analyzing N-isotope mass balances in natural systems.
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