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Measuring in vivo protein half-life.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2011
Author Bojkowska Karolina, Santoni de Sio Francesca, Barde Isabelle, Offner Sandra, Verp Sonia, Heinis Christian, Johnsson Kai, Trono Didier,
Project KRAB/KAP1 epigenetic regulation in the control of memory and emotional traits: from mice to humans.
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Original article (peer-reviewed)

Journal Chemistry & biology
Volume (Issue) 18(6)
Page(s) 805 - 15
Title of proceedings Chemistry & biology
DOI 10.1016/j.chembiol.2011.03.014

Abstract

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.
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