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Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Zamolo Susanna J., Darbre Tamis, Reymond Jean-Louis,
Project Chemical Space Design of Small Molecules and Peptides
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Original article (peer-reviewed)

Journal Chemical Communications
Volume (Issue) 56(80)
Page(s) 11981 - 11984
Title of proceedings Chemical Communications
DOI 10.1039/d0cc04750c

Open Access

Type of Open Access Publisher (Gold Open Access)


There is currently a lack of efficient reagents to transfect cells with large plasmid DNA, which would be enabling tools for gene editing using CRISPR/Cas9 technology. Herein, we report the discovery of peptide dendrimer Z22 as a non-viral vector for transfecting large CRISPR/Cas9 pDNA into 3D-tumor spheroids with exceptionally high efficiency, low cytotoxicity and low immunogenicity.