Publication

Back to overview

Growth and characterization of different human rhinovirus C types in three-dimensional human airway epithelia reconstituted in vitro.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Tapparel Caroline, Sobo Komla, Constant Samuel, Huang Song, Van Belle Sandra, Kaiser Laurent, Tapparel Caroline, Sobo Komla, Constant Samuel, Huang Song, Van Belle Sandra, Kaiser Laurent,
Project Rhinovirus et entérovirus: déterminants génomiques et phénotypes associés
Show all

Original article (peer-reviewed)

Journal Virology
Volume (Issue) 446(1-2)
Page(s) 1 - 8
Title of proceedings Virology
DOI 10.1016/j.virol.2013.06.031

Abstract

New molecular diagnostic tools have recently allowed the discovery of human rhinovirus species C (HRV-C) that may be overrepresented in children with lower respiratory tract complications. Unlike HRV-A and HRV-B, HRV-C cannot be propagated in conventional immortalized cell lines and their biological properties have been difficult to study. Recent studies have described the successful amplification of HRV-C15, HRV-C11, and HRV-C41 in sinus mucosal organ cultures and in fully differentiated human airway epithelial cells. Consistent with these studies, we report that a panel of clinical HRV-C specimens including HRV-C2, HRV-C7, HRV-C12, HRV-C15, and HRV-C29 types were all capable of mediating productive infection in reconstituted 3D human primary upper airway epithelial tissues and that the virions enter and exit preferentially through the apical surface. Similar to HRV-A and HRV-B, our data support the acid sensitivity of HRV-C. We observed also that the optimum temperature requirement during HRV-C growth may be type-dependent.
-