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Signal Control by Self-Assembly of Fluorophores in a Molecular Beacon – A Model Study.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2011
Author Biner Sarah M., Kummer Dominic, Malinovskii Vladimir L., Häner Robert,
Project DNA-assisted self-organization of aromatic chromophores
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Original article (peer-reviewed)

Journal Org. Biomol. Chem.
Volume (Issue) 9
Page(s) 2628 - 2633
Title of proceedings Org. Biomol. Chem.
DOI 10.1039/C0OB01132K

Abstract

Pyrene excimer fluorescence is efficiently regulated by formation of pi-stacked aggregates between dialkynylpyrenes and PDI residues located in the stem region of the MB. By varying the numbers of pyrene (Y) and PDI (E) residues we could show that the two types of chromophores interact in a donor-acceptor fashion in the closed form. The formation of various types of complexes (EYEY → YEY → EY) is revealed by characteristic changes in CD, UV/Vis and fluorescence spectra. Conformational changes induced by target recognition leads to direct pyrene-pyrene interaction and, thus, efficient excimer fluorescence. The data show that signal control is an intrinsic property of the interacting chromophores. Therefore, the concept described herein, represents a functional module that may find applications not only in MBs but also in other input/output systems using light as the source of information.
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