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Meta- and Orthogonal Integration of Influenza "OMICs" Data Defines a Role for UBR4 in Virus Budding.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Tripathi Shashank, Pohl Marie O, Zhou Yingyao, Rodriguez-Frandsen Ariel, Wang Guojun, Stein David A, Moulton Hong M, DeJesus Paul, Che Jianwei, Mulder Lubbertus C F, Yángüez Emilio, Andenmatten Dario, Pache Lars, Manicassamy Balaji, Albrecht Randy A, Gonzalez Maria G, Nguyen Quy, Brass Abraham, Elledge Stephen, White Michael, Shapira Sagi, Hacohen Nir, Karlas Alexander, Meyer Thomas F, Shales Michael, Gatorano Andre, Johnson Jeffrey R, Jang Gwen, Johnson Tasha, Verschueren Erik, Sanders Doug, Krogan Nevan, Shaw Megan, König Renate, Stertz Silke, García-Sastre Adolfo, Chanda Sumit K,
Project The role of phosphorylation events during influenza A virus entry
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Original article (peer-reviewed)

Journal Cell host & microbe
Page(s) 723 - 35
Title of proceedings Cell host & microbe
DOI 10.1016/j.chom.2015.11.002

Open Access

URL https://www.sciencedirect.com/science/article/pii/S1931312815004564?via%3Dihub
Type of Open Access Green OA Embargo (Freely available via Repository after an embargo)

Abstract

Several systems-level datasets designed to dissect host-pathogen interactions during influenza A infection have been reported. However, apparent discordance among these data has hampered their full utility toward advancing mechanistic and therapeutic knowledge. To collectively reconcile these datasets, we performed a meta-analysis of data from eight published RNAi screens and integrated these data with three protein interaction datasets, including one generated within the context of this study. Further integration of these data with global virus-host interaction analyses revealed a functionally validated biochemical landscape of the influenza-host interface, which can be queried through a simplified and customizable web portal (http://www.metascape.org/IAV). Follow-up studies revealed that the putative ubiquitin ligase UBR4 associates with the viral M2 protein and promotes apical transport of viral proteins. Taken together, the integrative analysis of influenza OMICs datasets illuminates a viral-host network of high-confidence human proteins that are essential for influenza A virus replication.
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