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Functional surface engineering by nucleotide-modulated potassium channel insertion into polymer membranes attached to solid supports.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Kowal Justyna Ł, Kowal Julia K, Wu Dalin, Stahlberg Henning, Palivan Cornelia G, Meier Wolfgang P,
Project Electron Microscopy of Membrane Proteins
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Original article (peer-reviewed)

Journal Biomaterials
Volume (Issue) 35(26)
Page(s) 7286 - 94
Title of proceedings Biomaterials
DOI 10.1016/j.biomaterials.2014.05.043

Abstract

Planar solid-supported membranes based on amphiphilic block copolymers represent promising systems for the artificial creation of structural surfaces. Here we introduce a method for engineering functional planar solid-supported membranes through insertion of active biomolecules. We show that membranes based on poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) amphiphilic diblock copolymers, which mimic natural membranes, are suitable for hosting biomolecules. Our strategy allows preparation of large-area, well-ordered polymer bilayers via Langmuir-Blodgett and Langmuir-Schaefer transfers, and insertion of biomolecules by using Bio-Beads. We demonstrate that a model membrane protein, the potassium channel from the bacterium Mesorhizobium loti, remains functional after insertion into the planar solid-supported polymer membrane. This approach can be easily extended to generate a platform of functional solid-supported membranes by insertion of different hydrophobic biomolecules, and employing different types of solid substrates for desired applications.
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