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Callose deposition: a multifaceted plant defense response.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Luna Estrella, Pastor Victoria, Robert Jérôme, Flors Victor, Mauch-Mani Brigitte, Ton Jurriaan,
Project Characterization of arabidopsis mutants altered in their ability to express BABA-induced resistance (BABA-IR) and priming
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Original article (peer-reviewed)

Journal Molecular plant-microbe interactions : MPMI
Volume (Issue) 24(2)
Page(s) 183 - 93
Title of proceedings Molecular plant-microbe interactions : MPMI
DOI 10.1094/MPMI-07-10-0149

Abstract

Callose deposition in Arabidopsis has emerged as a popular model system to quantify activity of plant immunity. However, there has been a noticeable rise in contradicting reports about the regulation of pathogen-induced callose. To address this controversy, we have examined the robustness of callose deposition under different growth conditions and in response to two different pathogen-associated molecular patterns, the flagellin epitope Flg22 and the polysaccharide chitosan. Based on a commonly used hydroponic culture system, we found that variations in growth conditions have a major impact on the plant's overall capacity to deposit callose. This environmental variability correlated with levels of hydrogen peroxide (H₂O₂) production. Depending on the growth conditions, pretreatment with abscissic acid stimulated or repressed callose deposition. Despite a similar effect of growth conditions on Flg22- and chitosan-induced callose, both responses showed differences in timing, tissue responsiveness, and colocalization with H₂O₂. Furthermore, mutant analysis revealed that Flg22- and chitosan-induced callose differ in the requirement for the NADPH oxidase RBOHD, the glucosinolate regulatory enzymes VTC1 and PEN2, and the callose synthase PMR4. Our study demonstrates that callose is a multifaceted defense response that is controlled by distinct signaling pathways, depending on the environmental conditions and the challenging pathogen-associated molecular pattern.
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