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Phosphorylation state-dependent modulation of spinal glycine receptors alleviates inflammatory pain.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Acuña Mario A, Yévenes Gonzalo E, Ralvenius William T, Benke Dietmar, Di Lio Alessandra, Lara Cesar O, Muñoz Braulio, Burgos Carlos F, Moraga-Cid Gustavo, Corringer Pierre-Jean, Zeilhofer Hanns Ulrich,
Project Dorsal Horn Neuronal Circuits Processing Itch
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Original article (peer-reviewed)

Journal The Journal of clinical investigation
Volume (Issue) 126(7)
Page(s) 2547 - 60
Title of proceedings The Journal of clinical investigation
DOI 10.1172/jci83817

Open Access

URL https://www.jci.org/articles/view/83817/pdf
Type of Open Access Website

Abstract

Diminished inhibitory neurotransmission in the superficial dorsal horn of the spinal cord is thought to contribute to chronic pain. In inflammatory pain, reductions in synaptic inhibition occur partially through prostaglandin E2- (PGE2-) and PKA-dependent phosphorylation of a specific subtype of glycine receptors (GlyRs) that contain α3 subunits. Here, we demonstrated that 2,6-di-tert-butylphenol (2,6-DTBP), a nonanesthetic propofol derivative, reverses inflammation-mediated disinhibition through a specific interaction with heteromeric αβGlyRs containing phosphorylated α3 subunits. We expressed mutant GlyRs in HEK293T cells, and electrophysiological analyses of these receptors showed that 2,6-DTBP interacted with a conserved phenylalanine residue in the membrane-associated stretch between transmembrane regions 3 and 4 of the GlyR α3 subunit. In native murine spinal cord tissue, 2,6-DTBP modulated synaptic, presumably αβ heteromeric, GlyRs only after priming with PGE2. This observation is consistent with results obtained from molecular modeling of the α-β subunit interface and suggests that in α3βGlyRs, the binding site is accessible to 2,6-DTBP only after PKA-dependent phosphorylation. In murine models of inflammatory pain, 2,6-DTBP reduced inflammatory hyperalgesia in an α3GlyR-dependent manner. Together, our data thus establish that selective potentiation of GlyR function is a promising strategy against chronic inflammatory pain and that, to our knowledge, 2,6-DTBP has a unique pharmacological profile that favors an interaction with GlyRs that have been primed by peripheral inflammation.
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