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Distinct domains of the AVRPM3 A2/F2 avirulence protein from wheat powdery mildew are involved in immune receptor recognition and putative effector function

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author McNally Kaitlin Elyse, Menardo Fabrizio, Lüthi Linda, Praz Coraline Rosalie, Müller Marion Claudia, Kunz Lukas, Ben-David Roi, Chandrasekhar Kottakota, Dinoor Amos, Cowger Christina, Meyers Emily, Xue Mingfeng, Zeng Fangsong, Gong Shuangjun, Yu Dazhao, Bourras Salim, Keller Beat,
Project Molecular analysis of three different types of fungal disease resistance mechanisms in cereals
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Original article (peer-reviewed)

Journal New Phytologist
Volume (Issue) 218(2)
Page(s) 681 - 695
Title of proceedings New Phytologist
DOI 10.1111/nph.15026

Open Access

URL http://doi.org/10.1111/nph.15026
Type of Open Access Publisher (Gold Open Access)

Abstract

Recognition of the AVRPM3A2/F2 avirulence protein from powdery mildew by the wheat PM3A/F immune receptor induces a hypersensitive response after co-expression in Nicotiana benthamiana. The molecular determinants of this interaction and how they shape natural AvrPm3a2/f2 allelic diversity are unknown. We sequenced the AvrPm3a2/f2 gene in a worldwide collection of 272 mildew isolates. Using the natural polymorphisms of AvrPm3a2/f2 as well as sequence information from related gene family members, we tested 85 single-residue-altered AVRPM3A2/F2 variants with PM3A, PM3F and PM3FL456P/Y458H (modified for improved signaling) in Nicotiana benthamiana for effects on recognition. An intact AvrPm3a2/f2 gene was found in all analyzed isolates and the protein variant recognized by PM3A/F occurred globally at high frequencies. Single-residue alterations in AVRPM3A2/F2 mostly disrupted, but occasionally enhanced, the recognition response by PM3A, PM3F and PM3FL456P/Y458H . Residues enhancing hypersensitive responses constituted a protein domain separate from both naturally occurring polymorphisms and positively selected residues of the gene family. These results demonstrate the utility of using gene family sequence diversity to screen residues for their role in recognition. This approach identified a putative interaction surface in AVRPM3A2/F2 not polymorphic in natural alleles. We conclude that molecular mechanisms besides recognition drive AvrPm3a2/f2 diversification.
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