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Lysine transporters in human trypanosomatid pathogens.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2012
Author Inbar Ehud, Canepa Gaspar E, Carrillo Carolina, Glaser Fabian, Suter Grotemeyer Marianne, Rentsch Doris, Zilberstein Dan, Pereira Claudio A,
Project Functional genomics of nutrient transporters in Trypanosoma brucei: From physiology to pharmacology
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Original article (peer-reviewed)

Journal Amino acids
Volume (Issue) 42(1)
Page(s) 347 - 60
Title of proceedings Amino acids
DOI 10.1007/s00726-010-0812-z

Abstract

In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively.
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