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Multi-panel drugs detection in human serum for personalized therapy

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author 34. Sandro Carrara Andrea Cavallini Victor Erokhin Giovanni De Micheli,
Project Innovative Enabling Micro-Nano-Bio-technologies for Implantable systems in molecular medicine and personalized therapy
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Original article (peer-reviewed)

Journal Biosensors and Bioelectronics
Page(s) 3914
Title of proceedings Biosensors and Bioelectronics


This work focuses on P450 biosensors based on multiwalled carbon nanotubes (MWCNT) and differ- ent cytochrome isoforms: 3A4, 2B4, 2C9. The proposed biosensors exhibit enhanced sensitivities and decreased detection limits thanks to carbon nanotubes. The MWCNT structuring improves the sen- sitivity from 5.1 to 20.5nA/mMmm2 in case of CYP2B4-mediated Benzphetamine detection, from 0.26 to 0.63 nA/􏱝M mm2 in case of CYP3A4-mediated Cyclophosphamide detection, and from 0.11 to 0.25 nA/􏱝M mm2 in case of CYP2C9-mediated Naproxen detection. By using MWCNT, the limit of detec- tion was enhanced from 59 to 12 􏱝M in case of Cyclophosphamide and from to 187 to 82 􏱝M in case of Naproxen. This makes possible the drug detection in human serum within the pharmacological range. In the paper, a new mathematical model is also proposed to succeed in discriminating different drug contri- butions in a mixture containing both Cyclophosphamide and Dextromethorphan or combining Naproxen and Flurbiprofen. Data analysis shows variations in reduction peaks that are dependent on the drug ratio, and that are consistent with competitive kinetics of substrates. This new approach enables multiple drug detection and opens the way to possible applications in personalized therapy