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A tRNA half modulates translation as stress response in Trypanosoma brucei

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Fricker Roger, Brogli Rebecca, Luidalepp Hannes, Wyss Leander, Fasnacht Michel, Joss Oliver, Zywicki Marek, Helm Mark, Schneider André, Cristodero Marina, Polacek Norbert,
Project Stress-mediated effects on ribosome functions and translation control
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Original article (peer-reviewed)

Journal Nature Communications
Volume (Issue) 10(1)
Page(s) 118 - 118
Title of proceedings Nature Communications
DOI 10.1038/s41467-018-07949-6

Open Access

Type of Open Access Publisher (Gold Open Access)


In the absence of extensive transcription control mechanisms the pathogenic parasite Trypanosoma brucei crucially depends on translation regulation to orchestrate gene expression. However, molecular insight into regulating protein biosynthesis is sparse. Here we analyze the small non-coding RNA (ncRNA) interactome of ribosomes in T. brucei during different growth conditions and life stages. Ribosome-associated ncRNAs have recently been recognized as unprecedented regulators of ribosome functions. Our data show that the tRNAThr 3 ´half is produced during nutrient deprivation and becomes one of the most abundant tRNAderived RNA fragments (tdRs). tRNAThr halves associate with ribosomes and polysomes and stimulate translation by facilitating mRNA loading during stress recovery once starvation conditions ceased. Blocking or depleting the endogenous tRNAThr halves mitigates this stimulatory effect both in vivo and in vitro. T. brucei and its close relatives lack the welldescribed mammalian enzymes for tRNA half processing, thus hinting at a unique tdR biogenesis in these parasites.