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A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2015
Author Dudin Omaya, Bendezu Felipe, Groux Raphael, Laroche Thierry, Seitz Arne, Martin Sophie,
Project Cell polarization in response to intra- and extracellular cues in fission yeast
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Original article (peer-reviewed)

Journal Journal of Cell Biology
Volume (Issue) 208
Page(s) 897
Title of proceedings Journal of Cell Biology
DOI doi/10.1083/jcb.201411124

Abstract

Cell–cell fusion is essential for fertilization. For fusion of walled cells, the cell wall must be degraded at a precise location but maintained in surrounding regions to protect against lysis. In fission yeast cells, the formin Fus1, which nucleates linear actin filaments, is essential for this process. In this paper, we show that this formin organizes a specific actin structure—the actin fusion focus. Structured illumination microscopy and livecell imaging of Fus1, actin, and type V myosins revealed an aster of actin filaments whose barbed ends are focalized near the plasma membrane. Focalization requires Fus1 and type V myosins and happens asynchronously always in the M cell first. Type V myosins are essential for fusion and concentrate cell wall hydrolases, but not cell wall synthases, at the fusion focus. Thus, the fusion focus focalizes cell wall dissolution within a broader cell wall synthesis zone to shift from cell growth to cell fusion.
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